Publication:20190625151934
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| Publication | |
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| URL | https://www.mdpi.com/2072-6694/11/6/839 |
| Title | EV-Associated miRNAs from Peritoneal Lavage are a Source of Biomarkers in Endometrial Cancer
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| Authors | Berta Roman-Canal, Cristian Pablo Moiola, Sònia Gatius, Sarah Bonnin, Maria Ruiz-Miró, Esperanza González, Xavier González-Tallada, Ivanna Llordella, Isabel Hernández, José M. Porcel, Antonio Gil-Moreno, Juan M. Falcón-Pérez, Julia Ponomarenko, Xavier Matias-Guiu, Eva Colas |
| Date | 2019-06
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| Publisher | Cancers |
| DOI | 10.3390/cancers11060839 |
| Tag | endometrial cancer, uterine cancer, exosomes, biomarkers, miRNAs, ascitic fluid, peritoneal lavage, liquid biopsy, extracellular vesicles, microRNAs |
Abstract:
Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology. Development of novel approaches that would permit us to improve the clinical management of EC patients is an unmet need. In this study, we investigate a novel approach to identify highly sensitive and specific biomarkers of EC using extracellular vesicles (EVs) isolated from the peritoneal lavage of EC patients. EVs of peritoneal lavages of 25 EC patients were isolated and their miRNA content was compared with miRNAs of EVs isolated from the ascitic fluid of 25 control patients. Expression of the EV-associated miRNAs was measured using the Taqman OpenArray technology that allowed us to detect 371 miRNAs. The analysis showed that 114 miRNAs were significantly dysregulated in EC patients, among which eight miRNAs, miRNA-383-5p, miRNA-10b-5p, miRNA-34c-3p, miRNA-449b-5p, miRNA-34c-5p, miRNA-200b-3p, miRNA-2110, and miRNA-34b-3p, demonstrated a classification performance at area under the receiver operating characteristic curve (AUC) values above 0.9. This finding opens an avenue for the use of EV-associated miRNAs of peritoneal lavages as an untapped source of biomarkers for EC.
Endometrial cancer (EC) is the sixth most common cancer in women worldwide and is responsible for more than 89,000 deaths every year. Mortality is associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis at an advanced stage, with a high grade and/or an aggressive histology. Development of novel approaches that would permit us to improve the clinical management of EC patients is an unmet need. In this study, we investigate a novel approach to identify highly sensitive and specific biomarkers of EC using extracellular vesicles (EVs) isolated from the peritoneal lavage of EC patients. EVs of peritoneal lavages of 25 EC patients were isolated and their miRNA content was compared with miRNAs of EVs isolated from the ascitic fluid of 25 control patients. Expression of the EV-associated miRNAs was measured using the Taqman OpenArray technology that allowed us to detect 371 miRNAs. The analysis showed that 114 miRNAs were significantly dysregulated in EC patients, among which eight miRNAs, miRNA-383-5p, miRNA-10b-5p, miRNA-34c-3p, miRNA-449b-5p, miRNA-34c-5p, miRNA-200b-3p, miRNA-2110, and miRNA-34b-3p, demonstrated a classification performance at area under the receiver operating characteristic curve (AUC) values above 0.9. This finding opens an avenue for the use of EV-associated miRNAs of peritoneal lavages as an untapped source of biomarkers for EC.
| Annotation | This publication in a high-impact cancer research journal is the result of a collaboration of the Biocore with the cancer researchers from the Vall D'hebron Hospital. This paper demonstrates that miRNAs isolated from extracellular vesicles from the peritoneal lavage can serve as biomarkers in endometrial cancer. Topics: cancer, biomarker discovery, translational medicine. Impact Factor 6,2. |
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